72 Ebola

72.1 Strategy To Stop Ebola Transmission

Marta 23/10/18

Pillars of Care:

  • Case Management
  • Case Finding, Contact Tracing
  • Safe and Dignified Burials (Particularly infectious once death, high viral burden). You need big community involvement
  • Social Mobilisation. Your strategy needs to work with the community that are effected. Needs engagement

72.1.1 Case Management

This is safe care for patients, but also safety for staff.

Staff safety was essential as initially many were dying from Ebola. But you need some patient engagement, as they were dying for lack of IV fluids/Abx, etc.

  • IPC guidance (PPE/Hand Washing/Etc)
  • Effective care based on clinical guidelines
  • Consider special populations (Pregnant, Paediatrics, HIV)
  • Consider how care appears to family members (is the ETU killing off patients?)
  • Aspects of laboratory use (double protection of the samples to allow transport)

Gene Xpert gives ebola results within 90 minutes

Q: Give a major presenting cause of reduced GCS in Ebola A: Hypoglycaemia

72.1.2 Case Finding

Steps:

  1. Alerted of case
  2. Investigate the case
  3. Trace contacts
  4. Monitor contacts for 3 weeks

If quarantine is enacted, you need to arrange that patients have food supply, you need to arrange that patients have someone that will look after their land and animals. Else they will not follow quarantine.

Patients had a single number to contact if they thought they/someone else had ebola. But then there were no ambulances to pick people up! So citizens didn’t trust the government and hotline, as no ambulances to take people to centres.

Q: Why do you try and trace contacts in an outbreak? A: Catching cases early reduces disease transmission and increases chance of individual survival

72.1.3 Safe Burial

Q: When are the two riskiest moments after death in Ebola transmission?

A: - Preparation of the body (washing the body) - The funeral ceremony itself (touching/hugging/kissing the body)

Needed engagement w/ burial teams through allowing families choice in how the ceremony works.

Some West African funerals/rites go on for several days

You need specific “burial teams” and they also need PPE

72.1.4 Social Mobilisation

Really an aspect of all other pillars. Needed for everything.

72.2 Virology & Diagnostics

Colin Brown, Naomi Walker

Enveloped -ve ssRNA virus

Really small genome (19kb)

Seven structural proteins (entry, genome, reproduction, transcription, host interferon resopnse, reproduction). Really simple virus, nothing there that should make it be latent (but it is!)

Most ebola tests are for the glycoprotein outer

Behaves like other -ve SSRNA viruses when it comes to entering cells. Goes to brain kidney pancreas liver, but really likes monocytes, neutrophils, NK cells. It kills NK cells, inactivates neutrophils, prevents macrophages from producing IFN. Increases levels of TNF IL-6. Attacks dendritic cells and turns of IFN response, preventing signalling to CD4 T cells.

It’s got an early proinflammatory stage initially, causing endothelial leakage. This is what causes fluid loss and bleeding.

When it attacks organs it’s got a direct toxic affect on these organs.

And it downregulates immune response.

You get a really high viraemia. The higher the viraemia, the more likely you are to die.

72.2.1 Principles of Diagnostics

Diagnostic Pathway:

  1. Assess against the case definition ->
  2. Meets the case definition ->
  3. Diagnostic test against body fluid (usually blood) ->
  4. Consider what the result means depending on how good the test is:

This can split you into:

  • Confirmed EVD
  • Possible EVD (for further testing)
  • No EVD

You might end up as Possible EVD when:

  • Negative test but pre-test probability v high
  • Negative test but v early testing after symptom onset
  • Positive test when you think it was v unlikely

At all stages of the testing process you need to follow full biosafety precautions. This includes transporting samples to lab!

72.2.2 Testing Options

  • Viral Culture - only way that tells you if you’ve got “live virus”
  • PCR (NAAT) - GOLD STANDARD currently (sens/spec) tells you there a viral fragments there, so do you know if it’s live infectious virus or not?
  • EIA (immunoassay)
  • Xpert (rapid NAAT)
  • Lateral flow RDT (rapid POC)

72.2.2.1 Ideal Test

  • Sens spec
  • Cheap
  • Low tech
  • Rapid
  • Transmissibility/Prognosticates
  • Culturally approprate
  • Safe
  • Easy
  • Low intraoperator variability

72.2.2.2 PCR

  • Good sensitivity and specificity
  • Lab based
  • Hours to days
  • Good for viral load (sort of, uses a proxy measure called the Cycle Threshold)
  • Doesn’t tell you if virus alive or dead

You need to deactivate virus, extract genetics, then amplification. Can do loads of samples together, but is time consuming, and expensive, and need loads of infrastructure.

72.2.2.3 “Point of Care” PCR

  • Xpert platform
  • Still high sensitivity and specificity
  • Takes 4 hours to run
  • Looks at a couple of targets
  • Currently in use in DRC

Needs less infrastructure than old PCR

72.2.2.4 Antibody Tests

Variety of antibody tests that are useful, but not as useful as PCR.

Role is more to work out whether someone previously had ebola, rather than “do thy have ebola right now”. Helped identify all the asymptomatic ebola patients.

72.2.2.5 Lateral Flow Assays

Works like a pregnancy test. Some commercially available.

72.3 Community Engagement

Golden rule of community engagment, start where the community are. Don’t try and create new systems, use what the community already use and trust.

A good outreach strategy is:

  1. multi platform,
  2. that uses existing communication networks,
  3. that must be trusted,
  4. that must be targeted at your population (example is patients may not use twitter, but journalists love it),
  5. that is consistent!
  6. that is culturally appropriate
  7. that is in the right language,
  8. that meets your objectives
  9. dialogue based
  10. mutually supportive (verbal plus written/etc)

Often women use different social structures than men.

Communities often don’t really trust NGOs (~20% trust), they do trust community health workers

Key groups:

  • Religious leaders
  • Moto taxis

72.3.1 Techniques

  • Posters
  • Community Leaders
  • Radio/TV - Difficulty here is often linked to political groups
  • Social Media
  • Face To Face

72.4 Knowledge Attitudes Practice (KAP) Survey

Gold standard for working out if your intervention works.

So we see in Beni and Butembo > 90% know how to protect themselve from Ebola and where to go. Over 80% know what the symptoms are.

72.4.1 DRC Problems

The problems with Beni currently is the conflict setting. Patients refused to go to treatment centres and absconding. Thats how cases got to Beni in the first place. And how cases got to Tchemia, and Kalunguta, and Musienne, and Masereka.o

This is what we mean when we say communities decide on whether a strategy works or fails.

Contact tracing should increase over time, and as an outbreak goes on proportion from known contacts should increase. That’s not what we’re seeing in DRC. The proportion from outside known cases goes up and up.

Red Cross have found the communities think that this is created to interfere with elections, or ethnic cleansing. “Its deliberatly introduced by humanitarians to benefit the employees, they strategy of the doctors to make lots of money.”

There’s been a long history of violence and massacres in Beni of civilians. 13,000 have died in massacres between 2014-2016, thought by government sorted militias.

1/4 believe that ebola is not real

1/3 believe that ebola is fabricated for financial gain

Almost half in Beni believe it’s fabricated to destabilise the region

Opposition politicians are reporting this even further.

There is a whole industry on essential oils, and hemp chocolate, and general bullshit in America on selling “ebola cures”

72.4.1.1 Solutions

Blasting information at people doesn’t work, it has to be a conversation with people. And remember as an NGO person you may be seen as part of the “conspiricy to kill everyone”

You have to listen, you have to find out what people are talking about for rumoured causes and cures. These rumours will change week by week. You need to follow changes. You need to use anthropologists! Rapid anthroplogical surveys and summaries needed.

SSHAP (Social science and humanitarian action platform)

Community engagement is needed in all pillars of the response. You need to feedback to all operational decision makers, they need to take it into account.

It takes about 6 weeks for a community to come to terms with having Ebola, after encountering it for first time.

Your relationship with the community starts the second you turn up.

Just cos a community isn’t engaging with your response doesn’t mean they aren’t responding! They’re doing things themselves. Limiting physical contact, barbers visibly disinfecting, roadside stalls offering handwashing, churches are offering services, communities are monitorine who’s coming in, they’re using plastic bags to protect it.

Top Three Community Demands Currently in DRC:

  • Better Overall Healthcare
  • WASH Supplies: Soap, Disinfectant
  • Blanket Vaccinations - Why are only contacts getting it? Why not me?
  • Explain Who You Are? Who’s the Ebola response team.

72.5 Organisation at the Ebola Treatment Centre and Screening

Ebola holding units, to group patients who are suspected ebola. These came out of the 2014 epidemic to try and break transmission, taking “maybe ebola” patients out of community.

The point is to isolate patients, keep staff and patients safe, provide good clinical care to patients.

How do you acheive this?

  • Limit access to patients and contaminated areas
  • Control movement of staff
  • Disinfection practices
  • Safe disposal of waste
  • Safe handling of bodies

72.5.1 Zones

Green Zone versus Red Zone

Green: No risk activities should happen here. (Supporting activites, Medical Staff, Cleaning Staff, Family members not at risk)

Red: Patients, Protected Staff, Waste, Morgue, Lab, Patients’ houses and vehicles. ALso should includ screening and disinfection.

Other areas: Training, counselling, rest, food, meeting.

72.5.2 Screening Area

This should be built to safely screen a large number of patients. Paitents may come by themselves, or they may be sent by an ambulance/family member/taxi.

72.5.2.1 Principles

You want complete separation betwen patient and health worker, no possibility of worker to cross over to patient side, unless wearring PPE. When healh care worker physically separated, not needed full PPE

Patient should be able to access their zone from outside, and have direct access to suspect and probable wards.

Physical separation should be at least 1.5 metres. This is done in a gap

72.5.2.2 Precautions of Screening

  • Green zone staff use gown, face protection, and gloves
  • “Red Zone” hygeinists in full PPE will disinfect the patient side after patient leaves.
  • No physical exam performed
  • Instruct patient how to use digital thermometer themselves

72.5.2.3 Flow

Patients should move from least to most contaminated zone. You should never go back the way.

Low risk -> Suspected -> Probable -> Confirmed -> Ward

Separate entrance for staff than patients

72.5.2.4 In the High Risk Zone

“Keep calm, cool, collected” 3C’s

You can’t run, can’t treat anything as emergency, can’t lose your own perspective of whats going on

  • Work with a buddy
  • Limited time inside
  • Plan what you’re doing before you go in.
  • What you take in does not come out

72.6 PPE

Marta

Ebola doesn’t live too long in inorganic material, but can be transmitted by any bodily fluid (blood first, then any other fluid).

You have to be very mindful of what you have touched, and how you hands close to face.

The more important phase of PPE is not putting it on, it’s taking it off.

There is not one true form of PPE for Ebola, multiple equal strategies.

Prior to West African Ebola outbreak, there were very little use of gloves/gowns/etc in Sierra Leone. Now the culture has completely changed.

Care of EVD Patients:

  • Scrubs and Boots
  • Face Protection to prevent splashes (face shield/goggles, and mask)
  • Gloves (double: inner and outer)
  • Gown/Coverall to cover clothing/exposed skin: The gown is more for screening area, the coverall is more for patient care
  • Head Cover/Hood
  • Disposable Waterproof Apron

For heavy cleaning/dead body management:

  • Rubber gloves as outer pair (thicker)
  • Reusable Apron (thicker)

Boots:

Waterproof plastic/rubber boots by all healthcare workers.

If no boots, then closed shoes plus shoe covers

Gloves:

Double. Need to be right size.

The outer glove should have a long cuff, ideally to mid-forearm.

Suit:

Needs to be balance of impermeability versus temperature and sweat. The more impermable the more you sewat and uncomfortable.

Head/Hair Protection:

Seperate hood?

  • Freedom of Movement = +
  • When removing PPE leads to exposed face = -

Mask:

In general any basic surgical mask that covers mouth and nose is safe. The worry with paper masks when they get wet, you sweat, and the mask becomes more porous.

So if you use a surgical mask, you need something else as well.

Goggles/Face Shield:

Problems with goggles is they fog super easily. You can wash them and decontaminate them easily

The face shields can get knocked off very easily, but convenient with air flow etc.

Apron:

These can be disposable or waterproof

72.6.1 Common Mistakes

Coverall - wrists can be exposed with wide moement

Don’t try to fix it by taping the gloves. What happens when you try to take it off?

Fix the gown with a loop to someithng

Masks - non structured masks can collapse against mouth

Goggles - when they fog so much you cannot see, don’t touch the goggles to adjust them! The face is the last thing you should touch. You can fix it with anti-fog spray or alcohol sanitiser.

72.7 Buddy System And Supervision

You never go in alone. Pair your new staff with more experienced ones.

First entry of unit: Just putting on PPE, walking through unit, doff, feedback

Directly supervise PPE every step of removal

Write name and function on gowns and time of entry

72.7.1 Inside ETU

No running, no emergency!

72.7.2 Glove Hygeine

  • Wash hands between patients
  • Change gloves between patients

72.7.3 Putting on PPE

  • No personal items
  • scrubs and boots
  • move to clean area
  • gather PPE
  • perform hand hygeine
  • floow donning sequence

Routine:

  • first pair of gloves
  • then coverall, with the gloves under the cuff of it
  • make thumb hole in PPE coverall to stop it riding down arm
  • then face mask on
  • then goggles/face shield
  • then hood up / or put on separate hood / or face shiled and bonnet
  • put on apron
  • second pair of gloves
  • check yourself in the mirror
  • get buddy to check you

Routine Off:

I’m contaminated, anything could be contaminated

No rush, do it SLOWLY

My gloves and my apron are the most contaminated bit

  • Listen to assessor
  • Stop and ask if any doubt
  • Let hygeinist know if PPE soiled

  • Exit w buddy
  • Step into bucket of 0.5% chlorine
  • Wash outer gloves for ONE MINUTE - 0.5% chlorine (that’s too strong for skin, will burn, but fine for this)
  • Remove apron, break at shoulder and back, don’t touch front as contaminated.
  • Wash outer goves again for another minute - 0.5% chlorine

  • Now if using separate hood/bonnet, remove it before removing coverall. Close eyes as removing hood
  • Remove coverall whilst trying not to touch anything
  • After freeing houlder remove outer gloves and put it on the floor
  • Wash inner gloves with 0.5%
  • Remove goggles from the back
  • Wash inner gloves with 0.5% chlorine
  • Remove gloves
  • Wash boots
  • Wash hands with water and soap or 0.05% chlorine. Wash until elbow.

Remember to divide disposable with reusable.

YOU NEED TO PROTECT YOUR FACE, THIS IS THE WAY YOU WILL GET INFECTED. DON’T WASH YOUR FACE AS YOU GET OUT!

72.8 EVD Survivors

Naomi Walker (26/10/18)

72.8.1 Ebola Complications

Most major complication of ebola is often Stigma! (This can also affect health care workers)

There have been loads of studies of ebola symptoms in survivors, but v few have controls (only two). You need to try and control for baseline illness, and not lots of these trials have done this. They still found loads of the same symptoms though.

72.8.1.1 Common

  • Mental health
  • Neurological (headache most common, significant cerebral atrophy seen in disabled patients)
  • Hearing (less common than vision)
  • Vision (common, large amounts of disability, not really any opthalmologists)
  • Abdo (chronic pain, not well understoof)
  • MSK
  • Sexual Health

Most common is joint pain, then headache, then muscle ache, then sleep disturbance

Most common serious complication would be eye symptoms

Other more common in survivors include:

  • retroorbital pain
  • fatigue
  • difficulty swallowing

72.8.1.2 Acute

72.8.1.2.1 Eye Complications:

They definitely increase in ebola survivors:

  • Uveitis
  • Conjunctivitis
  • Cataract
  • Scar/Ulcers

Inflammation in the eye is common, and 1/4 can be blind in that eye

72.8.1.3 Pathogenesis

Why are survivors getting these?

  • Is it continued immune activation?
  • Is it immune complex deposition? (you get it in Dengue)

  • Do patients with more severe disease, get more complications?
  • Patients with immunomodulatory therapy seem to have higher rates of complications. Is it that the severe patients who would have died otherwise, are now surviving with complications?

72.8.1.4 Delayed Viral Clearance

There is viable virus in “cured” ebola patients in immunological protected patients:

  • Semen/Testicles
  • Eyes
  • CNS
  • Placenta/Fetus
  • Joints

In some results it seems to have persisted in semen in up to 18 months! Gone in 3/4 of patients by 7-9 months. But this is PCR findings, who knows if the virus is alive or not? You need viral culture for that.

72.8.1.4.1 Eye

One american with severe illness, who required significant supportive management and novel therapies. Got a pan uveitis 10 weeks after “recovering” from the illness. Intraocular aspirate was positive on PCR (Tears and Blood negative).

72.8.1.4.2 CNS

30 yr patient who was recovering in a testing unit, had become PCR negative. Then became drowsy and confused on 16 days. Her CSF was positive, as was her sweat. But her blood was negative.

39 year nurse who was severely unwell, then 9 months later developed a meningoencephalitis.

72.8.1.4.3 Placenta and Fetus

Delivery of a stillborn infant who was ebola positive, to a mother who had only ever had mild illness and never treated in a treatment centre. Mother turned out to be antibody positive.

There have been secondary cases of ebola virus from delivery of these still births.

72.8.1.4.4 Relapse?

We don’t really know what the chat is here. There are few (unpublished) case reports of people ?relapsing (positive test and symptoms, negative test no symptoms, positive test and symptoms), they have all been HIV positive so far.

72.8.2 WHO Guidelines

There are national guidelines in Sierra Leone. They also had financial support for survivors, free health care for survivors. UNfortunately a lot of that funding is now gone.

72.8.3 Infection Prevention and Control in survivors

When treating survivors, check temperature, no examining witth bare hands according to WHO

72.8.3.1 Pregnancy and Childbirth?

  • Full PPE is needed for delivery at childbirth of survivors (if ebola during pregnancy) (most likely will be stillbirth)